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Emergence of Multidrug-Resistant Mycobacterium Tuberculosis of the Beijing Lineage in Portugal and Guinea-Bissau: a Snapshot of Moving Clones by Whole-Genome Sequencing

2021Journal article Open access
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dc.contributor.authorPerdigão, J
dc.contributor.authorSilva, C
dc.contributor.authorMaltez, F
dc.contributor.authorMachado, D
dc.contributor.authorMiranda, A
dc.contributor.authorCouto, I
dc.contributor.authorRabna, P
dc.contributor.authorFlorez de Sessions, P
dc.contributor.authorPhelan, J
dc.contributor.authorPain, A
dc.contributor.authorMcNerney, R
dc.contributor.authorHibberd, M
dc.contributor.authorMokrousov, I
dc.contributor.authorClark, T
dc.contributor.authorViveiros, M
dc.contributor.authorPortugal, I
dc.date.accessioned2022-03-03T13:17:49Z
dc.date.available2022-03-03T13:17:49Z
dc.date.issued2021
dc.description.abstractThe Beijing genotype comprises a highly disseminated strain type that is frequently associated with multidrug resistant (MDR) tuberculosis (TB) and increased transmissibility but, countries such as Portugal and Guinea-Bissau fall outside the regions phylogeographically associated with this specific genotype. Nevertheless, recent data shows that this genotype might be gradually emerging in these two countries as an underlying cause of primary MDR-TB. Here, we describe the emergence of Mycobacterium tuberculosis Beijing strains associated with MDR-TB in Portugal and Guinea-Bissau demonstrating the presence of the well described superclusters 100-32 and 94-32 in Portugal and Guinea-Bissau, respectively. Genome-wide analysis and comparison with a global genomic dataset of M. tuberculosis Beijing strains, revealed the presence of two genomic clusters encompassing isolates from Portugal and Guinea-Bissau, GC1 (n = 121) and GC2 (n = 39), both of which bore SNP signatures compatible with the 100-32/B0/W148 and 94-32/Central Asia Outbreak clades, respectively. Moreover, GC2 encompasses a cross-border cluster between Portugal, Guinea-Bissau and Brazil thus supporting migration-associated introduction of MDR-TB and subsequent clonal expansion at the community-level. The comparison with global Beijing datasets demonstrates the global reach of the disease and its complex dissemination across multiple countries while in parallel there are clear microevolutionary trajectories towards extensively drug resistant TB.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationEmerg Microbes Infect. 2020 Dec;9(1):1342-1353.pt_PT
dc.identifier.doi10.1080/22221751.2020.1774425.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.17/3979
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTaylor & Francispt_PT
dc.subjectHCC INFpt_PT
dc.subjectBeijingpt_PT
dc.subjectBrazilpt_PT
dc.subjectDNA, Bacterial / genetics*pt_PT
dc.subjectDrug Resistance, Multiple, Bacterial*pt_PT
dc.subjectGuinea-Bissaupt_PT
dc.subjectHumanspt_PT
dc.subjectHigh-Throughput Nucleotide Sequencingpt_PT
dc.subjectMycobacterium tuberculosis / classification*pt_PT
dc.subjectMycobacterium tuberculosis / geneticspt_PT
dc.subjectMycobacterium tuberculosis / isolation & purificationpt_PT
dc.subjectPhylogenypt_PT
dc.subjectPortugalpt_PT
dc.subjectPhylogeographypt_PT
dc.subjectTuberculosis, Multidrug-Resistant / microbiology*pt_PT
dc.subjectWhole Genome Sequencing / methods*pt_PT
dc.titleEmergence of Multidrug-Resistant Mycobacterium Tuberculosis of the Beijing Lineage in Portugal and Guinea-Bissau: a Snapshot of Moving Clones by Whole-Genome Sequencingpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1353pt_PT
oaire.citation.startPage1342pt_PT
oaire.citation.titleEmerging Microbes & Infectionspt_PT
oaire.citation.volume9pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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