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Comparison of Nasopharyngeal Samples for SARS‐CoV ‐2 Detection in a Paediatric Cohort

2021Journal article Open access
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dc.contributor.authorRodrigues, J
dc.contributor.authorGouveia, C
dc.contributor.authorSantos, MA
dc.contributor.authorCosta, O
dc.contributor.authorCôrte‐Real, R
dc.contributor.authorBrito, MJ
dc.date.accessioned2022-05-09T14:13:11Z
dc.date.available2022-05-09T14:13:11Z
dc.date.issued2021
dc.description.abstractAim: The diagnosis of coronavirus disease 2019 (COVID-19) depends on accurate and rapid testing. Choosing an appropriate sample may impact diagnosis. Naso-oropharyngeal swabs (NOS) are most frequently used, despite several limitations. Since studies suggest nasopharyngeal aspirate (NPA) as a superior alternative in children, we hypothesised collecting both nasopharyngeal swab and aspirate would improve our diagnostic accuracy. Methods: Observational, longitudinal, prospective study from 7 March to 7 May in a tertiary paediatric hospital in Lisbon. The objective was to compare the rate of detection of SARS-CoV-2 between NOS and NPA samples collected simultaneously. Results: A total of 438 samples collected from 85 patients with confirmed COVID-19. There were 47.7% overall positive specimens - 32% (70/219) positive NOS and 63.5% (139/219) positive NPA. The tests were 67.6% concordant (k = 0.45). 50.3% had positive NPA with negative NOS, while 1.3% had positive NOS with negative NPA. NPA proved to be more sensitive (98.6% with 95% confidence interval 91.2-99.9% vs. 49.6% with 95% confidence interval 41.1-58.2%, P < 0.001). Additionally, the difference between NPA and NOS positive samples was statistically significant across all population groups (age, health condition, clinical presentation, contact with COVID-19 patients or need for hospitalisation), meaning NPA is more sensitive overall. Conclusions: Nasopharyngeal aspirates had greater sensitivity than naso-oropharyngeal swabs in detecting SARS-CoV-2. Our results suggest paediatric patients would benefit from collecting nasopharyngeal aspirates in hospital settings, whenever feasible, to improve diagnosis of COVID-19.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Paediatr Child Health . 2021 Jul;57(7):1078-1081pt_PT
dc.identifier.doi10.1111/jpc.15405pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.17/4069
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.subjectCOVID-19.pt_PT
dc.subjectSARS-CoV-2pt_PT
dc.subjectMolecular biologypt_PT
dc.subjectPolymerase chain reactionpt_PT
dc.subjectHDE PAT CLINpt_PT
dc.subjectHDE INF PEDpt_PT
dc.titleComparison of Nasopharyngeal Samples for SARS‐CoV ‐2 Detection in a Paediatric Cohortpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1081pt_PT
oaire.citation.issue7pt_PT
oaire.citation.startPage1078pt_PT
oaire.citation.titleJournal of Paediatrics and Child Healthpt_PT
oaire.citation.volume57pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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