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Accuracy of the ELITe MGB Assays for the Detection of Carbapenemases, CTX-M, Staphylococcus Aureus and MecA/C Genes Directly From Respiratory Samples

2020-06Journal article Open access
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dc.contributor.authorBoattini, M
dc.contributor.authorBianco, G
dc.contributor.authorIannaccone, M
dc.contributor.authorCharrier, L
dc.contributor.authorAlmeida, A
dc.contributor.authorDe Intinis, G
dc.contributor.authorCavallo, R
dc.contributor.authorCosta, C
dc.date.accessioned2021-03-17T15:42:23Z
dc.date.available2021-03-17T15:42:23Z
dc.date.issued2020-06
dc.description.abstractIntroduction: Bacterial lower respiratory tract infections (BLRTI) may represent serious clinical conditions which can lead to respiratory failure, intensive care unit admission and high hospital costs. The detection of carbapenemase- and extended-spectrum β-lactamase (ESBL)-producing Enterobacterales, as well as meticillin-resistant Staphylococcus aureus (MRSA), has become a major issue, especially in healthcare-associated infections. This study aimed to determine whether molecular assays could detect genes encoding carbapenemases, ESBL and MRSA directly from respiratory samples in order to expedite appropriate therapy and infection control for patients with BLRTI. Methods: The carbapenem-resistant enterobacterales (CRE), ESBL and MRSA/SA ELITe MGB assays were performed directly on 354 respiratory specimens sampled from 318 patients admitted with BLRTI. Molecular results were compared with routine culture-based diagnostics results. Results: Positive (PPV) and negative (NPV) predictive values of the CRE ELITe MGB kit were 75.9% [95% confidence interval (CI) 60.3-86.7] and 100%, respectively. PPV and NPV of the ESBL ELITe MGB kit were 80.8% (95% CI 63.6-91.0) and 99.1% (95% CI 96.6-99.8), respectively. PPV and NPV of the MRSA/SA ELITe MGB kit were 91.7% (95% CI 73.7-97.7)/100% and 98.3% (95% CI 89.8-99.3)/96.8% (95% CI 81.6-99.5), respectively. Discussion: Validity assessment of molecular assays detecting the main antibiotic resistance genes directly from respiratory samples showed high accuracy compared with culture-based results. Molecular assays detecting the main carbapenemase, ESBL, S. aureus and meticillin resistance encoding genes provide an interesting tool with potential to expedite optimization of antibiotic therapy and infection control practices in patients with BLRTI.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Hosp Infect. 2020 Jun;105(2):306-310.pt_PT
dc.identifier.doi10.1016/j.jhin.2019.12.025pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.17/3598
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.subjectBacterial Loadpt_PT
dc.subjectBacterial Proteinspt_PT
dc.subjectBronchoalveolar Lavage Fluidpt_PT
dc.subjectColony Count, Microbialpt_PT
dc.subjectCross Infectionpt_PT
dc.subjectDrug Resistance, Bacterialpt_PT
dc.subjectHumanspt_PT
dc.subjectMicrobial Sensitivity Testspt_PT
dc.subjectMolecular Diagnostic Techniquespt_PT
dc.subjectPenicillin-Binding Proteinspt_PT
dc.subjectReproducibility of Resultspt_PT
dc.subjectRespiratory Systempt_PT
dc.subjectStaphylococcal Infectionspt_PT
dc.subjectStaphylococcus aureuspt_PT
dc.subjectBeta-Lactamasespt_PT
dc.subjectHSM MEDpt_PT
dc.titleAccuracy of the ELITe MGB Assays for the Detection of Carbapenemases, CTX-M, Staphylococcus Aureus and MecA/C Genes Directly From Respiratory Samplespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage310pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage306pt_PT
oaire.citation.titleJournal of Hospital Infectionpt_PT
oaire.citation.volume105pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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