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Quantitative Analysis of Immunoglobulin E Reactivity Profiles in Patients Allergic or Sensitized to Natural Rubber Latex (Hevea Brasiliensis)

2007-11Journal article Open access
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dc.contributor.authorRaulf-Heimsoth, M
dc.contributor.authorRihs, H-P
dc.contributor.authorRozynek, P
dc.contributor.authorCremer, R
dc.contributor.authorGaspar, A
dc.contributor.authorPires, G
dc.contributor.authorYeang, H
dc.contributor.authorArif, S
dc.contributor.authorHamilton, R
dc.contributor.authorSander, I
dc.contributor.authorLundberg, M
dc.contributor.authorBrüning, T
dc.date.accessioned2016-05-11T11:13:40Z
dc.date.available2016-05-11T11:13:40Z
dc.date.issued2007-11
dc.description.abstractCharacterized native and recombinant Hevea brasiliensis (rHev b) natural rubber latex (NRL) allergens are available to assess patient allergen sensitization profiles. OBJECTIVE: Quantification of individual IgE responses to the spectrum of documented NRL allergens and evaluation of cross-reactive carbohydrate determinants (CCDs) for more definitive diagnosis. METHODS: Sera of 104 healthcare workers (HCW; 51 German, 21 Portuguese, 32 American), 31 spina bifida patients (SB; 11 German, 20 Portuguese) and 10 Portuguese with multiple surgeries (MS) were analysed for allergen-specific IgE antibody (sIgE) to NRL, single Hev b allergens and CCDs with ImmunoCAP technology. RESULTS: In all patient groups rHev b 5-sIgE concentrations were the most pronounced. Hev b 2, 5, 6.01 and 13 were identified as the major allergens in HCW and combined with Hev b 1 and Hev b 3 in SB. In MS Hev b 1 displayed an intermediate relevance. Different sIgE antibody levels to native Hevea brasiliensis (nHev b) 2 and rHev b 6.01 allowed discrimination of SB with clinical relevant latex allergy vs. those with latex sensitization. Sensitization profiles of German, Portuguese and American patients were equivalent. rHev b 5, 6.01 and nHev b 13 combined detected 100% of the latex-allergic HCW and 80.1% of the SB. Only 8.3% of the sera showed sIgE response to CCDs. CONCLUSIONS: Hev b 1, 2, 5, 6.01 and 13 were identified as the major Hev b allergens and they should be present in standardized latex extracts and in vitro allergosorbents. CCDs are only of minor relevance in patients with clinical relevant latex allergy. Component-resolved diagnostic analyses for latex allergy set the stage for an allergen-directed immunotherapy strategypt_PT
dc.identifier.citationClin Exp Allergy. 2007 Nov;37(11):1657-67pt_PT
dc.identifier.doi10.1111/j.1365-2222.2007.02833.xpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.17/2480
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherBlackwell Publishing Ltdpt_PT
dc.subjectAntigen-Antibody Reactions/immunologypt_PT
dc.subjectCarbohydrates/immunologypt_PT
dc.subjectCarbohydrates/immunologypt_PT
dc.subjectHevea/chemistrypt_PT
dc.subjectImmunoglobulin E/immunologypt_PT
dc.subjectLatex Hypersensitivity/immunologypt_PT
dc.subjectRecombinant Proteins/biosynthesispt_PT
dc.subjectRubberpt_PT
dc.subjectSpinal Dysraphism/complicationspt_PT
dc.subjectAdolescentpt_PT
dc.subjectAdultpt_PT
dc.subjectChildpt_PT
dc.subjectChild, Preschoolpt_PT
dc.subjectHDE ALERpt_PT
dc.titleQuantitative Analysis of Immunoglobulin E Reactivity Profiles in Patients Allergic or Sensitized to Natural Rubber Latex (Hevea Brasiliensis)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1667pt_PT
oaire.citation.issue11pt_PT
oaire.citation.startPage1657pt_PT
oaire.citation.volume37pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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