Publication
Regulatory Cells, Cytokine Pattern and Clinical Risk Factors for Asthma in Infants and Young Children with Recurrent Wheeze
| dc.contributor.author | Borrego, LM | |
| dc.contributor.author | Arroz, MJ | |
| dc.contributor.author | Videira, P | |
| dc.contributor.author | Martins, C | |
| dc.contributor.author | Guimarães, H | |
| dc.contributor.author | Nunes, G | |
| dc.contributor.author | Papoila, AL | |
| dc.contributor.author | Trindade, H | |
| dc.date.accessioned | 2016-05-11T11:23:31Z | |
| dc.date.available | 2016-05-11T11:23:31Z | |
| dc.date.issued | 2009-08 | |
| dc.description.abstract | Several risk factors for asthma have been identified in infants and young children with recurrent wheeze. However, published literature has reported contradictory findings regarding the underlying immunological mechanisms. OBJECTIVES: This study was designed to assess and compare the immunological status during the first 2 years in steroid-naive young children with >or= three episodes of physician-confirmed wheeze (n=50), with and without clinical risk factors for developing subsequent asthma (i.e. parental asthma or a personal history of eczema and/or two of the following: wheezing without colds, a personal history of allergic rhinitis and peripheral blood eosinophilia >4%), with age-matched healthy controls (n=30). METHODS: Peripheral blood CD4(+)CD25(+) and CD4(+)CD25(high) T cells and their cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4), GITR and Foxp3 expression were analysed by flow cytometry. Cytokine (IFN-gamma, TGF-beta and IL-10), CTLA-4 and Foxp3 mRNA expression were evaluated (real-time PCR) after peripheral blood mononuclear cell stimulation with phorbol 12-myristate 13-acetate (PMA) (24 h) and house dust mite (HDM) extracts (7th day). RESULTS: Flow cytometry results showed a significant reduction in the absolute number of CD4(+)CD25(high) and the absolute and percentage numbers of CD4(+)CD25(+)CTLA-4(+) in wheezy children compared with healthy controls. Wheezy children at a high risk of developing asthma had a significantly lower absolute number of CD4(+)CD25(+) (P=0.01) and CD4(+)CD25(high) (P=0.04), compared with those at a low risk. After PMA stimulation, CTLA-4 (P=0.03) and Foxp3 (P=0.02) expression was diminished in wheezy children compared with the healthy children. After HDM stimulation, CTLA-4 (P=0.03) and IFN-gamma (P=0.04) expression was diminished in wheezy children compared with healthy children. High-risk children had lower expression of IFN-gamma (P=0.03) compared with low-risk and healthy children and lower expression of CTLA-4 (P=0.01) compared with healthy children. CONCLUSIONS: Although our findings suggest that some immunological parameters are impaired in children with recurrent wheeze, particularly with a high risk for asthma, further studies are needed in order to assess their potential as surrogate predictor factors for asthma in early life. | pt_PT |
| dc.identifier.citation | Clin Exp Allergy. 2009 Aug;39(8):1160-9 | pt_PT |
| dc.identifier.doi | 10.1111/j.1365-2222.2009.03253.x | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.17/2481 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Blackwell Publishing Ltd | pt_PT |
| dc.subject | Asthma/immunology | pt_PT |
| dc.subject | Cytokines/genetics | pt_PT |
| dc.subject | Cytokines/immunology | pt_PT |
| dc.subject | Flow Cytometry | pt_PT |
| dc.subject | Interferon-gamma/biosynthesis | pt_PT |
| dc.subject | Respiratory Sounds/immunology | pt_PT |
| dc.subject | Child | pt_PT |
| dc.subject | HDE ALER | pt_PT |
| dc.title | Regulatory Cells, Cytokine Pattern and Clinical Risk Factors for Asthma in Infants and Young Children with Recurrent Wheeze | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 1169 | pt_PT |
| oaire.citation.issue | 8 | pt_PT |
| oaire.citation.startPage | 1160 | pt_PT |
| oaire.citation.volume | 39 | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |