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Multicentre Study Highlighting Clinical Relevance of New High-Throughput Methodologies in Molecular Epidemiology of Pneumocystis Jirovecii Pneumonia

2016-06Journal article Open access
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dc.contributor.authorEsteves, F
dc.contributor.authorde Sousa, B
dc.contributor.authorCalderón, EJ
dc.contributor.authorHuang, L
dc.contributor.authorBadura, R
dc.contributor.authorMaltez, F
dc.contributor.authorBassat, Q
dc.contributor.authorde Armas, Y
dc.contributor.authorAntunes, F
dc.contributor.authorMatos, O
dc.date.accessioned2017-06-08T14:42:27Z
dc.date.available2017-06-08T14:42:27Z
dc.date.issued2016-06
dc.description.abstractPneumocystis jirovecii causes severe interstitial pneumonia (PcP) in immunosuppressed patients. This multicentre study assessed the distribution frequencies of epidemiologically relevant genetic markers of P. jirovecii in different geographic populations from Portugal, the USA, Spain, Cuba and Mozambique, and the relationship between the molecular data and the geographical and clinical information, based on a multifactorial approach. The high-throughput typing strategy for P. jirovecii characterization consisted of DNA pooling using quantitative real-time PCR followed by multiplex-PCR/single base extension. The frequencies of relevant P. jirovecii single nucleotide polymorphisms (mt85, SOD110, SOD215, DHFR312, DHPS165 and DHPS171) encoded at four loci were estimated in ten DNA pooled samples representing a total of 182 individual samples. Putative multilocus genotypes of P. jirovecii were shown to be clustered due to geographic differences but were also dependent on clinical characteristics of the populations studied. The haplotype DHFR312T/SOD110C/SOD215T was associated with severe AIDS-related PcP and high P. jirovecii burdens. The frequencies of this genetic variant of P. jirovecii were significantly higher in patients with AIDS-related PcP from Portugal and the USA than in the colonized patients from Portugal, and Spain, and children infected with P. jirovecii from Cuba or Mozambique, highlighting the importance of this haplotype, apparently associated with the severity of the disease and specific clinical groups. Patients from the USA and Mozambique showed higher rates of DHPS mutants, which may suggest the circulation of P. jirovecii organisms potentially related with trimethoprim-sulfamethoxazole resistance in those geographical regions. This report assessed the worldwide distribution of P. jirovecii haplotypes and their epidemiological impact in distinct geographic and clinical populations.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationClin Microbiol Infect. 2016 Jun;22(6):566.e9-566.e19pt_PT
dc.identifier.doi10.1016/j.cmi.2016.03.013pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.17/2695
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.subjectAdolescentpt_PT
dc.subjectAdultpt_PT
dc.subjectAgedpt_PT
dc.subjectCluster Analysispt_PT
dc.subjectFemalept_PT
dc.subjectHaplotypespt_PT
dc.subjectHumanspt_PT
dc.subjectMalept_PT
dc.subjectMiddle Agedpt_PT
dc.subjectMolecular Epidemiologypt_PT
dc.subjectMolecular Typingpt_PT
dc.subjectMultiplex Polymerase Chain Reactionpt_PT
dc.subjectMycological Typing Techniquespt_PT
dc.subjectPneumocystis cariniipt_PT
dc.subjectPneumonia, Pneumocystispt_PT
dc.subjectPolymorphism, Single Nucleotidept_PT
dc.subjectReal-Time Polymerase Chain Reactionpt_PT
dc.subjectYoung Adultpt_PT
dc.subjectHigh-Throughput Screening Assayspt_PT
dc.subjectHCC INFpt_PT
dc.titleMulticentre Study Highlighting Clinical Relevance of New High-Throughput Methodologies in Molecular Epidemiology of Pneumocystis Jirovecii Pneumoniapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-MIC%2F116716%2F2010/PT
oaire.citation.endPage566.e19pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage566.e9pt_PT
oaire.citation.titleClinical Microbiology and Infectionpt_PT
oaire.citation.volume22pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication709ab094-c9da-4cd2-9a5e-b93582484594
relation.isProjectOfPublication.latestForDiscovery709ab094-c9da-4cd2-9a5e-b93582484594

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