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Different in Vivo Reactivity Profile in Health Care Workers and Patients with Spina Bifida to Internal and External Latex Glove Surface-Derived Allergen Extracts

2012-03Journal article Open access
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dc.contributor.authorPeixinho, CM
dc.contributor.authorTavares-Ratado, P
dc.contributor.authorGabriel, MF
dc.contributor.authorRomeira, AM
dc.contributor.authorLozoya-Ibanez, C
dc.contributor.authorTaborda-Barata, L
dc.contributor.authorTomaz, CT
dc.date.accessioned2016-03-18T11:20:54Z
dc.date.available2016-03-18T11:20:54Z
dc.date.issued2012-03
dc.description.abstractBACKGROUND: Allergy to natural rubber latex is a well-recognized health problem, especially among health care workers and patients with spina bifida. Despite latex sensitization being acquired in health institutions in both health care workers and patients with spina bifida, differences in allergen sensitization profiles have been described between these two risk groups. OBJECTIVE: To investigate the in vivo reactivity of health care workers and patients with spina bifida to extracts of internal and external surfaces of latex gloves and also to specific extracts enriched in major allergens for these risk groups. METHODS: Gloves from different manufacturers were used for protein extraction, and salt precipitation and hydrophobic interaction chromatography (HIC) were applied to obtain the enriched latex extracts. The major latex allergens were quantified by an enzyme immunoassay. The extracts obtained were tested in 14 volunteers using skin prick tests (SPT). RESULTS: Latex glove extracts enriched in the hydrophobic allergens that are most often seen in patients with spina bifida were obtained by selective precipitation, whereas HIC produced extracts enriched in the hydrophilic allergens commonly found in health care workers. The health care workers had positive SPTs to glove extracts from internal surfaces and to the hydrophilic allergen-enriched extracts. By contrast, patients with spina bifida had larger skin reactions both to external glove extracts and to the extracts enriched with the hydrophobic major allergens for this risk group. Despite the protein concentration of these extracts being less than half the concentration of the commercial extract, the weal-and-flare reactions were of similar magnitude. CONCLUSION: Using novel latex extracts, our study showed a different in vivo reactivity pattern in health care workers and in patients with spina bifida to extracts of the internal and external surfaces of gloves, which suggests that sensitization may occur by different routes of exposure, and that this influences the allergen reactivity profiles of these risk groupspt_PT
dc.identifier.citationBr J Dermatol. 2012 Mar;166(3):518-24pt_PT
dc.identifier.doi10.1111/j.1365-2133.2011.10656.xpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.17/2435
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherBritish Association of Dermatologistspt_PT
dc.subjectAllergens/adverse effectspt_PT
dc.subjectAllergens/immunologypt_PT
dc.subjectChromatography, Liquid/methodspt_PT
dc.subjectHydrophobic and Hydrophilic Interactionspt_PT
dc.subjectImmunoglobulin E/metabolismpt_PT
dc.subjectGloves, Surgical/adverse effectspt_PT
dc.subjectLatex/adverse effectspt_PT
dc.subjectLatex/immunologypt_PT
dc.subjectLatex Hypersensitivity/immunologypt_PT
dc.subjectSpinal Dysraphism/immunologypt_PT
dc.subjectSkin Testspt_PT
dc.subjectFemalept_PT
dc.subjectMalept_PT
dc.subjectAdolescentpt_PT
dc.subjectAdultpt_PT
dc.subjectHDE ALERpt_PT
dc.titleDifferent in Vivo Reactivity Profile in Health Care Workers and Patients with Spina Bifida to Internal and External Latex Glove Surface-Derived Allergen Extractspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage524pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage518pt_PT
oaire.citation.volume166pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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