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Therapeutic Activity of Superoxide Dismutase-Containing Enzymosomes on Rat Liver Ischaemia-Reperfusion Injury Followed by Magnetic Resonance Microscopy

2017-11-15Journal article Open access
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dc.contributor.authorMarcelino, P
dc.contributor.authorMarinho, HS
dc.contributor.authorCampos, MC
dc.contributor.authorNeves, AR
dc.contributor.authorReal, C
dc.contributor.authorFontes, FS
dc.contributor.authorCarvalho, A
dc.contributor.authorFeio, G
dc.contributor.authorMartins, MB
dc.contributor.authorCorvo, ML
dc.date.accessioned2019-03-21T15:44:54Z
dc.date.available2019-03-21T15:44:54Z
dc.date.issued2017-11-15
dc.description.abstractLiver ischaemia-reperfusion injury (IRI) may occur during hepatic surgery and is unavoidable in liver transplantation. Superoxide dismutase enzymosomes (SOD-enzymosomes), liposomes where SOD is at the liposomal surface expressing enzymatic activity in intact form without the need of liposomal disruption, were developed with the aim of having a better insight into its antioxidant therapeutic outcome in IRI. We also aimed at validating magnetic resonance microscopy (MRM) at 7T as a tool to follow IRI. SOD-enzymosomes were characterized and tested in a rat ischaemia-reperfusion model and the therapeutic outcome was compared with conventional long circulating SOD liposomes and free SOD using biochemical liver injury biomarkers, histology and MRM. MRM results correlated with those obtained using classical biochemical biomarkers of liver injury and liver histology. Moreover, MRM images suggested that the therapeutic efficacy of both SOD liposomal formulations used was related to prevention of peripheral biliary ductular damage and disrupted vascular architecture. Therefore, MRM at 7T is a useful technique to follow IRI. SOD-enzymosomes were more effective than conventional liposomes in reducing liver ischaemia-reperfusion injury and this may be due to a short therapeutic window.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationEur J Pharm Sci. 2017 Nov 15;109:464-471.pt_PT
dc.identifier.doi10.1016/j.ejps.2017.09.008pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.17/3214
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.subjectAlanine Transaminasept_PT
dc.subjectAnimalspt_PT
dc.subjectAspartate Aminotransferasespt_PT
dc.subjectLiposomespt_PT
dc.subjectLiverpt_PT
dc.subjectMagnetic Resonance Spectroscopypt_PT
dc.subjectMalept_PT
dc.subjectMicroscopypt_PT
dc.subjectRats, Wistarpt_PT
dc.subjectReperfusion Injurypt_PT
dc.subjectSuperoxide Dismutasept_PT
dc.subjectTranscription Factor RelApt_PT
dc.subjectgamma-Glutamyltransferasept_PT
dc.subjectHCC ANPATpt_PT
dc.titleTherapeutic Activity of Superoxide Dismutase-Containing Enzymosomes on Rat Liver Ischaemia-Reperfusion Injury Followed by Magnetic Resonance Microscopypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage471pt_PT
oaire.citation.startPage464pt_PT
oaire.citation.titleEuropean Journal of Pharmaceutical Sciencespt_PT
oaire.citation.volume109pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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