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A Model of Free Tissue Transfer: The Rat Epigastric Free Flap

2017-01-15Journal article Open access
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dc.contributor.authorCasal, D
dc.contributor.authorPais, D
dc.contributor.authorIria, I
dc.contributor.authorMota-Silva, E
dc.contributor.authorAlmeida, MA
dc.contributor.authorAlves, S
dc.contributor.authorPen, C
dc.contributor.authorFarinho, A
dc.contributor.authorMascarenhas-Lemos, L
dc.contributor.authorFerreira-Silva, J
dc.contributor.authorFerraz-Oliveira, M
dc.contributor.authorVassilenko, V
dc.contributor.authorVideira, P
dc.contributor.authorGory O'Neill, J
dc.date.accessioned2018-01-31T16:24:21Z
dc.date.available2018-01-31T16:24:21Z
dc.date.issued2017-01-15
dc.description.abstractFree tissue transfer has been increasingly used in clinical practice since the 1970s, allowing reconstruction of complex and otherwise untreatable defects resulting from tumor extirpation, trauma, infections, malformations or burns. Free flaps are particularly useful for reconstructing highly complex anatomical regions, like those of the head and neck, the hand, the foot and the perineum. Moreover, basic and translational research in the area of free tissue transfer is of great clinical potential. Notwithstanding, surgical trainees and researchers are frequently deterred from using microsurgical models of tissue transfer, due to lack of information regarding the technical aspects involved in the operative procedures. The aim of this paper is to present the steps required to transfer a fasciocutaneous epigastric free flap to the neck in the rat. This flap is based on the superficial epigastric artery and vein, which originates from and drain into the femoral artery and vein, respectively. On average the caliber of the superficial epigastric vein is 0.6 to 0.8 mm, contrasting with the 0.3 to 0.5 mm of the superficial epigastric artery. Histologically, the flap is a composite block of tissues, containing skin (epidermis and dermis), a layer of fat tissue (panniculus adiposus), a layer of striated muscle (panniculus carnosus), and a layer of loose areolar tissue. Succinctly, the epigastric flap is raised on its pedicle vessels that are then anastomosed to the external jugular vein and to the carotid artery on the ventral surface of the rat's neck. According to our experience, this model guarantees the complete survival of approximately 70 to 80% of epigastric flaps transferred to the neck region. The flap can be evaluated whenever needed by visual inspection. Hence, the authors believe this is a good experimental model for microsurgical research and training.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Vis Exp. 2017 Jan 15;(119).pt_PT
dc.identifier.doi10.3791/55281pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.17/2875
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMyJove Corporationpt_PT
dc.subjectAdipose Tissuept_PT
dc.subjectAnimalspt_PT
dc.subjectCarotid Arteriespt_PT
dc.subjectEpigastric Arteriespt_PT
dc.subjectFemoral Arterypt_PT
dc.subjectFemoral Veinpt_PT
dc.subjectFree Tissue Flapspt_PT
dc.subjectJugular Veinspt_PT
dc.subjectMuscle, Skeletalpt_PT
dc.subjectRatspt_PT
dc.subjectSurgical Flapspt_PT
dc.subjectNeckpt_PT
dc.subjectCHLC CPRpt_PT
dc.subjectCHLC PAT CLINpt_PT
dc.titleA Model of Free Tissue Transfer: The Rat Epigastric Free Flappt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue119pt_PT
oaire.citation.titleJournal of Visualized Experimentspt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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