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BD-2 and BD-3 Increase Skin Flap Survival in a Model of Ischemia and Pseudomonas Aeruginosa Infection

2019-05-27Journal article Open access
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dc.contributor.authorCasal, D
dc.contributor.authorIria, I
dc.contributor.authorRamalho, JS
dc.contributor.authorAlves, S
dc.contributor.authorMota-Silva, E
dc.contributor.authorMascarenhas-Lemos, L
dc.contributor.authorPontinha, C
dc.contributor.authorGuadalupe-Cabral, M
dc.contributor.authorFerreira-Silva, J
dc.contributor.authorFerraz-Oliveira, M
dc.contributor.authorVassilenko, V
dc.contributor.authorGoyri-O'Neill, J
dc.contributor.authorPais, D
dc.contributor.authorVideira, P
dc.date.accessioned2019-05-30T11:43:58Z
dc.date.available2019-05-30T11:43:58Z
dc.date.issued2019-05-27
dc.description.abstractThe main aim of this work was to study the usefulness of human β-defensins 2 (BD-2) and 3 (BD-3), which are part of the innate immune system, in the treatment of infected ischemic skin flaps. We investigated the effect of transducing rat ischemic skin flaps with lentiviral vectors encoding human BD-2, BD-3, or both BD-2 and BD-3, to increase flap survival in the context of a P. aeruginosa infection associated with a foreign body. The secondary endpoints assessed were: bacterial counts, and biofilm formation on the surface of the foreign body. A local ischemic environment was created by producing arterialized venous flaps in the left epigastric region of rats. Flaps were intentionally infected by placing underneath them two catheters with 105 CFU of P. aeruginosa before the surgical wounds were hermetically closed. Flap biopsies were performed 3 and 7 days post-operatively, and the specimens submitted to immunohistochemical analysis for BD-2 and BD-3, as well as to bacterial quantification. Subsequently, the catheter segments were analyzed with scanning electron microscopy (SEM). Flaps transduced with BD-2 and BD-3 showed expression of these defensins and presented increased flap survival. Rats transduced with BD-3 presented a net reduction in the number of P. aeruginosa on the surface of the foreign body and lesser biofilm formation.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSci Rep. 2019 May 27;9(1):7854.pt_PT
dc.identifier.doi10.1038/s41598-019-44153-ypt_PT
dc.identifier.urihttp://hdl.handle.net/10400.17/3265
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNature Publishing Grouppt_PT
dc.subjectCHLC CPRpt_PT
dc.subjectCHLC PAT CLINpt_PT
dc.subjectAnimalspt_PT
dc.subjectAnti-Bacterial Agents / metabolismpt_PT
dc.subjectAnti-Bacterial Agents / therapeutic use
dc.subjectDisease Models, Animal
dc.subjectGenetic Therapy
dc.subjectGenetic Vectors / therapeutic use
dc.subjectGenetic Vectors / genetics
dc.subjectGraft Survival
dc.subjectIschemia / complications
dc.subjectPseudomonas Infections / complications
dc.subjectMale
dc.subjectPseudomonas Infections / therapy
dc.subjectPseudomonas aeruginosa / drug effects
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectSkin Transplantation / adverse effects
dc.subjectSurgical Flaps / adverse effects
dc.subjectSurgical Flaps / microbiology
dc.subjectTransduction, Genetic
dc.subjectbeta-Defensins / genetics
dc.subjectbeta-Defensins / therapeutic use
dc.titleBD-2 and BD-3 Increase Skin Flap Survival in a Model of Ischemia and Pseudomonas Aeruginosa Infectionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1pt_PT
oaire.citation.startPage7854pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume9pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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