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Susceptibility Patterns of Staphylococcus Aureus Biofilms in Diabetic Foot Infections

2016-06-23Journal article Open access
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dc.contributor.authorMottola, C
dc.contributor.authorMatias, C
dc.contributor.authorMendes, JJ
dc.contributor.authorMelo-Cristino, J
dc.contributor.authorTavares, L
dc.contributor.authorCavaco-Silva, P
dc.contributor.authorOliveira, M
dc.date.accessioned2016-11-10T16:07:24Z
dc.date.available2016-11-10T16:07:24Z
dc.date.issued2016-06-23
dc.description.abstractBACKGROUND: Foot infections are a major cause of morbidity in people with diabetes and the most common cause of diabetes-related hospitalization and lower extremity amputation. Staphylococcus aureus is by far the most frequent species isolated from these infections. In particular, methicillin-resistant S. aureus (MRSA) has emerged as a major clinical and epidemiological problem in hospitals. MRSA strains have the ability to be resistant to most β-lactam antibiotics, but also to a wide range of other antimicrobials, making infections difficult to manage and very costly to treat. To date, there are two fifth-generation cephalosporins generally efficacious against MRSA, ceftaroline and ceftobripole, sharing a similar spectrum. Biofilm formation is one of the most important virulence traits of S. aureus. Biofilm growth plays an important role during infection by providing defence against several antagonistic mechanisms. In this study, we analysed the antimicrobial susceptibility patterns of biofilm-producing S. aureus strains isolated from diabetic foot infections. The antibiotic minimum inhibitory concentration (MIC) was determined for ten antimicrobial compounds, along with the minimum biofilm inhibitory concentration (MBIC) and minimum biofilm eradication concentration (MBEC), followed by PCR identification of genetic determinants of biofilm production and antimicrobial resistance. RESULTS: Results demonstrate that very high concentrations of the most used antibiotics in treating diabetic foot infections (DFI) are required to inhibit S. aureus biofilms in vitro, which may explain why monotherapy with these agents frequently fails to eradicate biofilm infections. In fact, biofilms were resistant to antibiotics at concentrations 10-1000 times greater than the ones required to kill free-living or planktonic cells. The only antibiotics able to inhibit biofilm eradication on 50 % of isolates were ceftaroline and gentamicin. CONCLUSIONS: The results suggest that the antibiotic susceptibility patterns cannot be applied to biofilm established infections. Selection of antimicrobial therapy is a critical step in DFI and should aim at overcoming biofilm disease in order to optimize the outcomes of this complex pathology.pt_PT
dc.identifier.citationBMC Microbiol. 2016 Jun 23;16(1):119pt_PT
dc.identifier.doi10.1186/s12866-016-0737-0pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.17/2579
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherBioMed Centralpt_PT
dc.relationBACTERIAL CROSS-TALK IN BIOFILM-ENCLOSED MIXED POPULATIONS FROM DIABETIC FOOT INFECTION
dc.subjectHSM MEDpt_PT
dc.subjectAnti-Bacterial Agents/pharmacologypt_PT
dc.subjectBiofilms/drug effectspt_PT
dc.subjectBiofilms/growth & developmentpt_PT
dc.subjectCephalosporins/pharmacologypt_PT
dc.subjectDiabetic Foot/drug therapypt_PT
dc.subjectDiabetic Foot/microbiology
dc.subjectGentamicins/pharmacology
dc.subjectMethicillin-Resistant Staphylococcus aureus/drug effects
dc.subjectMethicillin-Resistant Staphylococcus aureus/genetics
dc.subjectMethicillin-Resistant Staphylococcus aureus/physiology
dc.subjectMicrobial Sensitivity Tests
dc.subjectPolymerase Chain Reaction/methods
dc.subjectStaphylococcal Infections/drug therapy
dc.subjectStaphylococcal Infections/microbiology
dc.subjectStaphylococcus aureus/drug effects
dc.subjectStaphylococcus aureus/genetics
dc.subjectStaphylococcus aureus/isolation & purification
dc.subjectStaphylococcus aureus/physiology
dc.subjectbeta-Lactams/pharmacology
dc.titleSusceptibility Patterns of Staphylococcus Aureus Biofilms in Diabetic Foot Infectionspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBACTERIAL CROSS-TALK IN BIOFILM-ENCLOSED MIXED POPULATIONS FROM DIABETIC FOOT INFECTION
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FCVT%2F00276%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-MIC%2F122816%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F72872%2F2010/PT
oaire.citation.issue1pt_PT
oaire.citation.startPage119pt_PT
oaire.citation.titleBMC Microbiologypt_PT
oaire.citation.volume16pt_PT
oaire.fundingStream5876
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationdec32f50-3617-46ec-bebd-ec767ad81165
relation.isProjectOfPublicationb6a49be4-4a5d-4a45-b73c-051ab15d3fb8
relation.isProjectOfPublicationc114a2c4-4e99-4862-bfd3-22d6c871a586
relation.isProjectOfPublication.latestForDiscoverydec32f50-3617-46ec-bebd-ec767ad81165

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