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Intracoronary Delivery of Human Mesenchymal/Stromal Stem Cells: Insights from Coronary Microcirculation Invasive Assessment in a Swine Model

2015Journal article Open access
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dc.contributor.authorFiarresga, A
dc.contributor.authorMata, M
dc.contributor.authorCavaco-Gonçalves, S
dc.contributor.authorSelas, M
dc.contributor.authorSimões, I
dc.contributor.authorOliveira, E
dc.contributor.authorCarrapiço, B
dc.contributor.authorCardim, N
dc.contributor.authorCabral, J
dc.contributor.authorCruz Ferreira, R
dc.contributor.authorSilva, C
dc.date.accessioned2016-03-18T17:40:56Z
dc.date.available2016-03-18T17:40:56Z
dc.date.issued2015
dc.description.abstractBACKGROUND: Mesenchymal stem/stromal cells have unique properties favorable to their use in clinical practice and have been studied for cardiac repair. However, these cells are larger than coronary microvessels and there is controversy about the risk of embolization and microinfarctions, which could jeopardize the safety and efficacy of intracoronary route for their delivery. The index of microcirculatory resistance (IMR) is an invasive method for quantitatively assessing the coronary microcirculation status. OBJECTIVES: To examine heart microcirculation after intracoronary injection of mesenchymal stem/stromal cells with the index of microcirculatory resistance. METHODS: Healthy swine were randomized to receive by intracoronary route either 30x106 MSC or the same solution with no cells (1% human albumin/PBS) (placebo). Blinded operators took coronary pressure and flow measurements, prior to intracoronary infusion and at 5 and 30 minutes post-delivery. Coronary flow reserve (CFR) and the IMR were compared between groups. RESULTS: CFR and IMR were done with a variance within the 3 transit time measurements of 6% at rest and 11% at maximal hyperemia. After intracoronary infusion there were no significant differences in CFR. The IMR was significantly higher in MSC-injected animals (at 30 minutes, 14.2U vs. 8.8U, p = 0.02) and intragroup analysis showed a significant increase of 112% from baseline to 30 minutes after cell infusion, although no electrocardiographic changes or clinical deterioration were noted. CONCLUSION: Overall, this study provides definitive evidence of microcirculatory disruption upon intracoronary administration of mesenchymal stem/stromal cells, in a large animal model closely resembling human cardiac physiology, function and anatomy.pt_PT
dc.identifier.citationPLoS One. 2015 Oct 19;10(10):e0139870.pt_PT
dc.identifier.doi10.1371/journal.pone.0139870pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.17/2441
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPlos Onept_PT
dc.relationCARDIOSAFER -Safety and efficacy of intracoronary mesenchymal stem/stromal cell (MSC)-based therapy for myocardial regeneration: a microcirculation study
dc.subjectHSM CARpt_PT
dc.subjectAnimalspt_PT
dc.subjectCoronary Circulationpt_PT
dc.subjectCoronary Vessels/cytologypt_PT
dc.subjectCoronary Vessels/physiology
dc.subjectHemodynamics
dc.subjectMesenchymal Stem Cell Transplantation
dc.subjectMicrocirculation
dc.subjectSwine
dc.titleIntracoronary Delivery of Human Mesenchymal/Stromal Stem Cells: Insights from Coronary Microcirculation Invasive Assessment in a Swine Modelpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCARDIOSAFER -Safety and efficacy of intracoronary mesenchymal stem/stromal cell (MSC)-based therapy for myocardial regeneration: a microcirculation study
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/EXPL%2FBIM-MED%2F1970%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH/BD/90001/2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH/BD/51572/2011/PT
oaire.citation.titlePlos Onept_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationb464c792-dd5f-4930-b76f-e36dd3a8d598
relation.isProjectOfPublication7a347fe0-b115-404e-8edc-875d83298023
relation.isProjectOfPublicationb0537789-0fc3-4531-9c94-b4c2ade7c219
relation.isProjectOfPublication.latestForDiscovery7a347fe0-b115-404e-8edc-875d83298023

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